Cryo‐EM on the Move: New Applications and Challenges

With the resolution revolution, cryo-electron microscopy (cryo-EM) has unveiled its potential in structural biology. It has now become the dominant method for solving de novo structures of macromolecular complexes. Approximately ten years after direct detection devices (DDD) became ubiquitously available, numerous laboratories have now integrated cryo-EM workflows in their publishing routines, and a plethora of landmark structures have been solved by single particle analysis. Advances in cryo-EM tomography, like FIB-milling, enable us to directly visualize macromolecular complexes within native cells at increasingly high resolution, opening the doors to entirely new applications in structural biology. Correlative light and electron microscopy workflows allow us to precisely target areas of interest and visualize molecular sociology in detail. Exciting new developments, including, for example, time-resolved structural studies, scanning transmission electron microscopy applications, and endogenously expressed protein-labelling techniques, are already around the corner and will soon be integrated into daily routines.

The seminar aims to provide an exchange platform for scientists at various career levels working on improving cryo-EM methodology. Therefore, we will discuss the latest software and hardware developments and tools to utilize this technology to its maximum. In keynote and invited talks, leading specialists and international experts will review this technology's present status and future applications. Ample time will be devoted to discussions and networking at poster sessions and flash talks for the participants.

The conference language will be English. The Wilhelm and Else Heraeus-Foundation bears the cost of full-board accommodation for all participants.